Which of the following is true of the sequence called the tata box?

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Q. Which of the following is true of the sequence called the TATA Box?

1. The sequence is located 30 base pairs downstream of the transcription site and is important for recognition by RNA polymerase.
2. The sequence is required for proper polyadenylation of the mRNA.
3. The sequence is located 30 base pairs upstream of the transcription site and is recognized by sigma factor.
4. The sequence is located 30 base pairs upstream of the transcription site and is important for recognition by RNA polymerase.

Answer: The sequence is located 30 base pairs upstream of the transcription site and is important for recognition by RNA polymerase.

"The TATA box (also called Goldberg-Hogness box) is a DNA sequence (cis-regulatory element) found in the promoter region of genes in archaea and eukaryotes; approximately 24% of human genes contain a TATA box within the core promoter."

Q. Which of the following best describes the significance of the TATA box in eukaryotic promoters

1. It is the recognition site for a specific transcription factor
2. it sets the reading frame of mRNA
3. it is the recognition site for ribosomal binding
4. its significance has not yet been determined

Answer: It is the binding site for a transcription factor.

Q. The ____________ of the eukaryotic promoter is analogous to the __________ of the bacterial promoter.

Answer: TATA box; -10 box

Q. What best describes a eukaryotic promoter?

1. It is found upstream of the gene and is where sigma proteins bind.
2. It is found downstream of the gene and is where sigma proteins bind.
3. It is found downstream of the gene and contains the TATA box.
4. It is found upstream of the gene and contains the TATA box.

Answer: It is found upstream of the gene and contains the TATA box.

Q. Which of the following is involved in the first step of initiating transcription?

1. RNA polymerase II
2. Mediator
3. TATA-binding protein (TBP)
4. Histone acetyltransferase (HAT)

Answer: TATA-binding protein (TBP)

The TATA box was first identified in 1978 as a component of eukaryotic promoters. Transcription is initiated at the TATA box in TATA-containing genes. The TATA box is the binding site of the TATA-binding protein (TBP) and transcription factors in some eukaryotic genes. Gene transcription by RNA polymerase II depends on the regulation of the core promoter by long-range regulatory elements such as enhancers and silencers. Without proper regulation of transcription, eukaryotic organisms would not be able to properly respond to their environment.

Goldberg-Hogness box (TATA Box)

›  A TATA box is a DNA sequence that indicates where a genetic sequence can be read and decoded.

›  It is a type of promoter sequence, which specifies to other molecules where transcription begins.

›  Transcription is a process that produces an RNA molecule from a DNA sequence.

›  The TATA box is named for its conserved DNA sequence, which is most commonly TATAAA.

›  Many eukaryotic genes have a conserved TATA box located 25-35 base pairs before the transcription start site of a gene.

›  The TATA box is able to define the direction of transcription and also indicates the DNA strand to be read.

›  Proteins called transcription factors can bind to the TATA box and recruit an enzyme called RNA polymerase, which synthesizes RNA from DNA.